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電気刺激法とカルシウムイオノファーによる卵子活性化に関する研究 The oocytes activation by electric pulse stimulation versus Calcium ionophore after Intracytoplasmic sperm injection.

年次大会 一般演題

2019年度 年次大会-一般演題 | 学術集会 - 一般演題(口頭発表)

発表者 : 藤江 周一朗,庄司 圭佑,三上 勇人,佐々木 雅弘

Abstract

【Purpose】
The present study was conducted to examine differences in the activation of human oocytes by electrical pulse versus the calcium ionophore.

【Methods】
The oocytes were activated electrically 30 min after ICSl.Oocytes, suspended in a phosphate-buffered saline were placed between two parallel electrodes in an electric chamber, and were subjected to a single, square DC pulse. Stimulated oocytes were immediately transferred back to HTF medium. The oocytes were exposed to A23187 for 30 min immediately transferred back to HTF medium.

【Results】
Seven hundred forty-one ICSI cycles were included in the study. The fertilization rate by was statistically significantly higher in the electro activated group as compared with in the the calcium ionophore(71% vs 48%). The oocyte degeneration rate was not statistically significantly different between the two groups(17% vs 28%).

【Conclusion】
The electric pulse activation seems to be more useful method than Calcium ionophore in cases of repeated failed fertilization after ICSI.

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